Generator
pRha-B0034
Part:BBa_K902067
Designed by: Himika Dastidar Group: iGEM12_Calgary (2012-10-02)
Rhamnose promoter with S7 micrococal nuclease
NOTE: This was our original submission of the part. The S7 micrococcal nuclease contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as K902084 in which we repaired the mutation. Please view the K902084 page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system.
This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |